Chemopreventive effects of Angelica gigas Nakai leaf (AGL), AGL kimchi and AGL added baechu kimchi were investigated. Kimchis were fermented at 15^oC for 1 day and then ripened at 5^oC to reach optimum pH of 4.3. The fermented kimchis were freeze-dried and extracted with methanol. AGL, AGL kimchi and 1% AGL added baechu kimchi exhibited higher inhibitory activities against the mutagenicities induced by aflatoxin B₁ (AFB₁) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100 than the baechu kimchi. Especially, AGL showed the highest inhibitory effect, and the AGL added baechu kimchi increased the antimutagenicities compared to baechu kimchi (p＜0.05). To investigate anticlastogenic effect of AGL and the kimchies in mice, supravital staining of micronucleus assay using mouse peripheral reticulocytes was performed. AGL revealed the highest anticlastogenic effect in mice against mitomycin C. Also, AGL and the AGL added kimchi were effective in reducing lung metastasis of colon 26-M3.1 cells. AGL kimchi and AGL added baechu kimchi increased chemopreventive effects of the kimchi and these results indicated that AGL addition increased chemopreventive effect of the kimchi.

Keywords: Kimchi, Angelica gigas Nakai, Ames test, Micronucleus assay, Metastasis