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Journal of Cancer Prevention


Journal of Korean Association of Cancer prevention 2003; 8(3): 160-165

Published online June 30, 2003

© Korean Society of Cancer Prevention

Differential Expressions of Gap Junction Proteins during Differentiation of Rat Neuronal Stem Cells

Se-Ran Yang1, Sung-Dae Cho1, Nam-Shik Ahn1, Ji-Won Jung1, Joon-Suk Park1, Nguyen Ba Tiep1, Ki-Su Park1, In-Sun Hong1, Eun-Hye Jo1, Min-Su Seo1, Byong-Su Yoon2, Yong-Soon Lee1 and Kyung-Sun Kang1


Gap junctional intercellular communication (GJIC) plays a key role during development, process of tissue differentiation, and in maintenance of adult tissue homeostasis. Neural stem cells leading to formation of cell clusters termed "neurospheres", can differentiate into neurons, oligodendrocytes, and astrocytes. We investigated the expression levels and distribution of connexin43 (Cx43) and connexin32 (Cx32), abundant gap junctional protein in neural cells and in neurospheres isolated from rat fetus embryonic day (ED) 17. During differentiation of neurospheres, expression of Cx43 and 32 were increased time-dependently within 72 h, and then decreased at 7 day in western blot analysis. TPA-induced inhibition of GJIC was confirmed by decreased fluorescence by SL/DT assay, and induced hyperphosphorylation of Cx43 while no changes in Cx32 levels in western blot assay. Our results indicate that GJIC may be a crucial role in the differentiation of neuronal stem cell. And this GJIC can be inhibited by TPA through the hyperphosphorylation of Cx43.

Keywords: Neurosphere, Stem cells, Gap junction, Cx43, Cx32, Differentiation

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