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Journal of Cancer Prevention

Journal of Korean Association of Cancer prevention 2000; 5(3): 144-153

Published online September 30, 2000

© Korean Society of Cancer Prevention

Effects of Mitomycin-C, Cisplatin, and Vinblastine
on Natural Killer Cell Activity in Mice

Heon Joong Kang, Kwang Hyuk Kim1, Hyun Yul Rhew,
and Kun-Young Park2

Abstract

Natural killer (NK) cells play a central role in immune surveillance against tumors and
viral infection. In this study, we investigated the effect of various clinically used
chemotherpeutic agents on NK cell activity. Splenic mononuclear cells from healthy
normal mice, C57BL/6, were tested for their cytotoxic activity in vitro in the presence
of mitomycin C, cisplatin, and vinblastine used for treatment of urologic tumors. Also,
normal mice were intravenously injected with these agents for in vivo tests. Using MTT
assay, natural killer (NK) cell-mediated growth inhibition of tumor cell was measured
in normal and agents-exposed groups. Non-adherent splenocytes of normal or agentsexposed
groups were cultured with Yac-1 cells at two different effector/target (E/T) cell
ratios of 200/1 and 100/1 in an assay volume of 0.2 ml. After the optical density of
culture wells containing MTT solution was measured at a wavelength of 540 nm, the
percentage of dead cells relative to the control target cell number was calculated.
Mitomycin-C and cisplatin suppressed the NK activity of mouse splenic mononuclear
cells in vitro, whereas vinblastine enhanced or suppressed its activity according to doses
with showing enhancement at 1.0 and 10.0 μg and suppression at 0.1 μg. In mice
injected with these agents, mitomycin-C and cisplatin enhanced the NK activity by twice
injections, vinblastine by once and three times injections. In contrast, its activity was
suppressed by once injection with mitomycin-C or cisplatin, and by twice injectons with
vinblastine. Therefore, the NK cell activity by exposure with anti-tumor agents shows
differences according to kinds and doses of drug, and injection number in vivo. It was
concluded that vinblastine had a higher effectiveness than mitomycin-C or cisplatin by
showing augmentation of NK cell activity both in vitro and in vivo

Keywords: Natural killer cell activity, Cisplatin, Mitomycin-C, Vinblastine, C57BL/6
mouse

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