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Journal of Cancer Prevention

Journal of Korean Association of Cancer prevention 2000; 5(3): 126-134

Published online September 30, 2000

© Korean Society of Cancer Prevention

Anti-clastogenicity Study of Ellagic Acid - In Vitro Chromosome Aberration
and Micronucleus Assay in Chinese Hamster Lung Fibroblast Cells and
In Vivo Micronucleus Assay in Bone Marrow Cells of ddY Mice

Hye Young Oh, Jong Won Kim, Chang Hwan Park1, Eui Sik Han,
Soo Jung Sohn, Il Hyun Kang, In Sook Kim and Kwang Won Ha

Abstract

Dietary anticarcinogens/mutagens have been demonstrated to significantly reduce the
incidence of cancers in animals and humans. The ellagic acid (EA) is a naturally occurring
plant phenol which has been shown to reduce the carcinogenicity and mutagenicity of a
variety of carcinogens, including N-nitrosomethylurea, benzo[a]pyrene, aflatoxin B1. The
chromosome aberration assay using cultured mammalian cells is one of the most sensitive
methods to predict environmental mutagens and/or carcinogens, and is a complementary
test to the Samonella/microsome assay (Ames test), and the in vitro micronucleus assay
is a mutagenicity test system for the detection of chemicals which induce the formation
of small membrane bound DNA fragment i.e. micronuclei in the cytoplasm of interphase
cells. The in vivo micronucleus assay, which examines metaphase chromosomes from the
bone marrow cells of rodents exposed to a test chemical just before sacrifice, is a powerful
tool and cytogenetic assay. This assay may be used to detect damage to actively dividing
cells not only from experimental test substances, but also from their metabolites as they
are formed within the test animal. In this study, EA was treated in chinese hamster lung
fibroblast (CHL) cells using in vitro chromosome aberration assay, in vitro micronucleus
assay, and in ddY mice using in vivo micronucleus assay for their inhibitory effects against
mitomycin C (MMC)-induced genotoxicity. The frequencies of chromosome aberrations
and micronuclei were found to be decreased following EA treatment. On the basis of our
results, we found that the EA had inhibitory effects on MMC-induced genotoxicity and we
concluded that these test systems are useful and sensitive for screening of antimutagens.

Keywords: Ellagic acid, Anti-clastogen, Anti-mutagen, Chromosome aberration
assay, In Vitro micronucleus assay, In vivo micronucleus assay

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