Experiments were undertaken to investigate the effect of cancer cell, sarcoma 180
(S 180) on production of interleukin(IL)-2, 4, and 10 in BALB/c mice. Splenocytes of
normal mice were incubated with S 180 cells. The culture supernatants were collected
at 2, 4, 6, 24, and 72 hr and assessed for IL-2, 4, and IL-10 production by
enzyme-linked immunosorbent assay. The production of IL-2 in the culture supernatant
of adherent cells exposed with S 180 was not detected, but it increased at 24 and 72
hr in culture with non-adherent or whole splenocyte. The production of IL-4 in the culture
supernatant of S 180, adherent, non-adherent, or whole splenocyte was detected, but
by co-culture with S 180 it showed significant increase at 72 hr. Also, the production
of IL-10 in the culture supernatant of S 180, adherent, non-adherent, or whole
splenocytes was detected. By exposure with S 180 it increased to 10-80 times in
comparison with the control. Normal mice were transplanted with S 180 into peritoneum.
Sera and peritoneal fluids(PF) were collected at 2, 4, 6, and 24 hr. The production of
IL-2 in sera was not detected, but in PF it was a little. The production of IL-4 in sera
or PF was detected, but it showed the significant decrease in comparison with the
control. The production of IL-10 in sera was detected in transplantation or control
group, and it increased in transplantation group. The production of IL-10 in PF showed
the increase at 24 hr. By exposure of S 180 tumor cells in immuno-competent cells
or transplantation with S 180 into mouse, IL-4 decreased in sera and PF, but IL-10
showed the remarkable increase in the culture supernatant of splenocytes and in the
sera. These data suggest that the unbalanced conditions such as suppression of IL-4
production and overproduction of IL-10 in vivo may be responsible for dysfunction of
immune system

Keywords: Mouse, Sarcoma 180, Interleukin-2, Interleukin-4, Interleukin-10