Journal of Korean Association of Cancer prevention 1998; 3(2): 115-121
Published online June 30, 1998
© Korean Society of Cancer Prevention
Hee-Jae Cha, Joo-Won Jeong, You Mie Lee, Motoharu Seiki1,
Ok Hee Kim2 and Kyu-Won Kim
During the steps of tumor metastasis, matrix metalloproteinase-9 (MMP-9) is
one of the most important enzymes and acts a key role to degrade the extracellular
matrix. Therefore suppressing the expression of MMP-9 may inhibit the tumor
invasion and metastasis. In this study, we developed the assay system to analyze
the expression of MMP-9 by constructing the MMP-9 reporter vector and confirming
the action of reporter protein by SEAP assay. Furthermore, we confirmed the
expression pattern of MMP-9 is similar to that of Northern blot and gelatin
zymogram assay. This assay system enables to detect the expression of MMP-9
by treating only simple methods. Therefore, we can apply this system to screen
the MMP-9 inhibitors in various candidate compounds and factors.
Keywords: MMP-9, SEAP vector, Invasion, Metastasis
Jeong Won Yun*, Dong Chul Kim*, Won Sup Lee, Jing Nan Lu, Min Jeong Kim, Myeong Hee Jung, Jin Hyun Kim, Dong Hoon Kim, Yung Hyun Choi, Gon Sup Kim, Chung Ho Ryu, Sung Chul Shin, Jin-Myung Jung, Soon Chan Hong, Gyung Hyuck Ko and Woo Song Hwa
Cancer prevention research 2009; 14(4): 315-321Mi-Sung Kim and Aree Moon
Journal of Korean Association of Cancer prevention 1999; 4(3): 119-126Taek Joon Yoon, Jae-Ho Do1 and Sung-Hoon Kim
Journal of Korean Association of Cancer prevention 2000; 5(1): 7-14