J Cancer Prev 2021; 26(2): 128-136
Published online June 30, 2021
https://doi.org/10.15430/JCP.2021.26.2.128
© Korean Society of Cancer Prevention
Eun-Ryeong Hahm1 , Su-Hyeong Kim1 , Sivapar V. Mathan2 , Rana P. Singh2 , Shivendra V. Singh1
1Department of Pharmacology & Chemical Biology, and UPMC Hillman Cancer Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA, 2Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India
Correspondence to :
Shivendra V. Singh, E-mail: singhs@upmc.edu, https://orcid.org/0000-0002-3733-144X
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Diallyl trisulfide (DATS), a metabolic by-product of processed garlic, is highly effective in inhibiting growth of human breast cancer cells in vitro and in vivo, but the underlying mechanisms are still not fully understood. In this study, we performed RNA-seq analyses using luminal-type (MCF-7) and basal-like (MDA-MB-231) human breast cancer cells to identify mechanistic targets of DATS. The Reactome Pathway Analysis revealed upregulation of genes associated with SLIT/ROBO tumor suppressor signaling following DATS treatment in both MCF-7 and MDA-MB-231 cells. However, the expression of SLIT2 and ROBO1 proteins or their downstream target C-X-C motif chemokine receptor 4 was not affected by DATS treatment in both cell lines. The Reactome as well as the Gene Ontology Pathways Analyses of the RNA-seq data from DATS-treated cells indicated downregulation of genes associated with G2/M phase cell cycle arrest in comparison with vehicle-treated control cells. Consistent with the RNA-seq data, DATS treatment caused a significant increase in the fraction of the G2/M population in both cell lines when compared to corresponding control cells. In addition, Ser10 phosphorylation of histone H3, a mitotic marker, was also increased significantly following DATS treatment in MCF-7 and MDA-MB-231 cells. These results indicate that while SLIT/ROBO signaling is not affected by DATS treatment, cell cycle arrest likely contributes to the antitumor effect of this phytochemical.
Keywords: Breast neoplasms, Allyl compounds, Sulfides, Chemoprevention
Eun-Ryeong Hahm, Shivendra V. Singh
J Cancer Prev 2022; 27(1): 22-30 https://doi.org/10.15430/JCP.2022.27.1.22Weidong Chen, Ga-Eun Lee, Dohyun Jeung, Jiin Byun, Wu Juan, Yong-Yeon Cho
J Cancer Prev 2023; 28(4): 143-149 https://doi.org/10.15430/JCP.2023.28.4.143Mark Steven Miller*, Altaf Mohammed*
J Cancer Prev 2023; 28(1): 24-28 https://doi.org/10.15430/JCP.2023.28.1.24