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Journal of Cancer Prevention

Original Article

Journal of Cancer Prevention 2017; 22(1): 33-39

Published online March 30, 2017

https://doi.org/10.15430/JCP.2017.22.1.33

© Korean Society of Cancer Prevention

Differential MicroRNA Expression Between Gastric Cancer Tissue and Non-cancerous Gastric Mucosa According to Helicobacter pylori Status

Jung Won Lee1,2, Nayoung Kim1,3, Ji Hyun Park3, Hee Jin Kim1,4, Hyun Chang1, Jung Min Kim5, Jin-Wook Kim1,3, and Dong Ho Lee1,3

1Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea, 2Department of Internal Medicine, Samsung Changwon Hospital, Changwon, Korea, 3Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea, 4Department of Internal Medicine, Myongji Hospital, Goyang, Korea, 5NAR Center, Inc., Daejeon Oriental Hospital of Daejeon University, Daejeon, Korea

Correspondence to :
Nayoung Kim, Department of Internal Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro 173beon-gil, Bundang-gu, Seongnam 13620, Korea, Tel: +82-31-787-7008, Fax: +82-31-787-4051, E-mail: nayoungkim49@empas.com, ORCID: Nayoung Kim, http://orcid.org/0000-0002-9397-0406

Received: March 6, 2017; Revised: March 15, 2017; Accepted: March 15, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

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Background

MicroRNAs (miRNAs) are key post-translational mechanisms which can regulate gene expression in gastric carcinogenesis. To identify miRNAs responsible for gastric carcinogenesis, we compared expression levels of miRNAs between gastric cancer tissue and non-cancerous gastric mucosa according to Helicobacter pylori status.

Methods

Total RNA was extracted from the cancerous regions of formalin-fixed, paraffin-embedded tissues of H. pylori-positive (n = 8) or H. pylori-negative (n = 8) patients with an intestinal type of gastric cancer. RNA expression was analyzed using a 3,523 miRNA profiling microarray based on the Sanger miRBase. Validation analysis was performed using TaqMan miRNA assays for biopsy samples from 107 patients consisted of control and gastric cancer with or without H. pylori. And then, expression levels of miRNAs were compared according to subgroups.

Results

A total of 156 miRNAs in the aberrant miRNA profiles across the miRNA microarray showed differential expression (at least a 2-fold change, P < 0.05) in cancer tissue, compared to noncancerous mucosa in both of H. pylori-negative and -positive samples. After 10 promising miRNAs were selected, validations by TaqMan miRNA assays confirmed that two miRNAs (hsa-miR-135b-5p and hsa-miR-196a-5p) were significantly increased and one miRNA (hsa-miR-145-5p) decreased in cancer tissue compared to non-cancerous gastric mucosa at H. pylori-negative group. For H. pylori-positive group, three miRNAs (hsa-miR-18a-5p, hsa-miR-135b-5p, and hsa-miR-196a-5p) were increased in cancer tissue. hsa-miR-135b-5p and hsa-miR-196a-5p were increased in gastric cancer in both of H. pylori-negative and -positive.

Conclusions

miRNA expression of the gastric cancer implies that different but partially common gastric cancer carcinogenic mechanisms might exist according to H. pylori status.

Keywords: Gastric cancer, microRNAs, Helicobacter pylori, Microarray

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