Article Search

Journal of Cancer Prevention

Original Article

Journal of Cancer Prevention 2016; 21(4): 249-256

Published online December 30, 2016

© Korean Society of Cancer Prevention

Sorbus rufopilosa Extract Exhibits Antioxidant and Anticancer Activities by Inducing Cell Cycle Arrest and Apoptosis in Human Colon Adenocarcinoma HT29 Cells

You Na Oh1, Soojung Jin1,2, Hyun-Jin Park1, Hyun Ju Kwon1,2, and Byung Woo Kim1,2

1Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan, Korea, 2Department of Life Science and Biotechnology, College of Natural Sciences and Human Ecology, Dong-Eui University, Busan, Korea

Correspondence to :
Byung Woo Kim, Blue-Bio Industry Regional Innovation Center, Dong-Eui University, 176 Eomgwang-ro, Busanjin-gu, Busan 47340, Korea, Tel: +82-51-890-2900, Fax: +82-505-182-6951, E-mail:

Received: November 23, 2016; Revised: December 15, 2016; Accepted: December 15, 2016

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



Sorbus rufopilosa, a tsema rowan, is a species of the small ornamental trees in the genus Sorbus and the family Rosaceae found in East Asia. The bioactivities of S. rufopilosa have not yet been fully determined. The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells.


To examine the antioxidant activity of EESR, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay was performed. Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis.


EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. EESR-induced apoptosis was associated with the upregulation of p53, a death receptor Fas, and a pro-apoptotic protein Bax and the activation of caspase 3, 8, and 9, resulting in the degradation of PARP.


EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. EESR may be a possible candidate for the anticancer drug development.

Keywords: Anticancer, Antioxidant, Apoptosis, Cell cycle arrest, Sorbus rufopilosa

Share this article on :

Related articles in JCP

Most KeyWord