To investigated the inhibitory effect of Jeju ground water on adipogenesis, 3T3-L1 preadipocyte cells were cultured and differentiated in media made by Jeju ground water and deionized water (DW) containing a mixture of dexamethasone, isobutylmethylxanthine (IBMX) and insulin. Oil Red O staining showed that lipid accumulation attenuated in adipocyte cells treated with Jeju ground water. The mRNA expression levels of peroxisome-activated receptorՃ (PPARՃ) and CCAAT-enhancer binding proteinՁ (C/EBPՁ), which are a key components of the transcriptional control of adipogenesis, were decreased on adipocyte expose to Jeju ground water. Furthermore, the mRNA expression levels of target genes of these factors, such as adipocyte fatty acid (aP2), lipoprotein lipase (LPL) and Leptin, were also decreased by Jeju ground water treatment compared with control water. These results suggest that Jeju ground water containing vanadium components suppressed 3T3-L1 adipocyte differentiation (Cancer Prev Res 16, 231-237, 2011)

Keywords: Jeju ground water, Adipogenesis, Adipocyte differentiation, Lipid