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Journal of Cancer Prevention

Original Article

Cancer prevention research 2011; 16(3): 200-209

Published online September 30, 2011

© Korean Society of Cancer Prevention

Gallotannin (GT) Regulates Apoptosis and Inflammation via PI3-kinase/p38 MAP Kinase Pathway in MDA-MB-231 Human Breast Cancer Cells

Seon-Mi Yu and Song-Ja Kim


Gallotannin (GT) is a type of tannic acid derived from plant polyphenol, it is usually involved in plant defense mechanism. GT has been used in medical agents such as anti-cancer, anti-inflammatory, anti-virial, anti-vacterial and anti-parastic in a wide range of cells. We have investigated the effects of GT on apoptosis and inflammation in MDA-MB-231 human breast cancer cells. Treatment of GT induced apoptosis and reduced proliferation as detected by MTT assay, and increased the expression of active caspase-3 as determined by Western blot analysis. Also, treatment of GT increased the expression of cyclooxygenase-2 (COX-2) and the production of prostaglandin E2 (PGE2) in a dose- and time-dependent manner as determined by Western blot analysis and PGE2 assay. We confirmed that GT dramatically caused phosphorylation of Akt and p38 kinase. Inhibition of PI3-kinase and p38 kinase with LY294002 and SB203580 prevented GT-induced apoptosis and COX-2 expression. Our results suggested that GT regulates apoptosis and COX-2 expression through PI3-kinase and p38 kinase pathway in MDA-MB-231 human breast cancer cells. (Cancer Prev Res 16, 200-209, 2011)

Keywords: Gallotannin (GT), Apoptosis, Cyclooxygenase-2 (COX-2), PI3-kinase, p38 kinase

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