Article Search

Journal of Cancer Prevention

Original Article

Cancer prevention research 2008; 13(2): 130-136

Published online June 30, 2008

© Korean Society of Cancer Prevention

Growth Inhibition of Human Hepatocarcinoma Cells by Water Extract of Euphorbiae humifusae was Associated with the Inhibition of Prostglandin E2 and NO Synthesis

Sang Ho Seo, Dong Yeok Shin1, Su Hyun Hong1, Sang Eun Park, Won Il Kim,Dong Il Park, Yung Hyun Choi1,2 and Sang Hoon Hong


Euphorbiae humifusae wind is a traditional medicinal plant generally used in Oriental medicine therapy. Previous studies have shown that E. humifusae exhibits anti-proliferative and antioxidant activities. However, the mechanism responsible for the anticancer effects of E. humifusae not clearly understood. In the present study, we investigated the anti-proliferative effects by the water extract of E. humifusae (WEEH) in Hep3B human hepatocarcinoma cells. It was found that WEEH could inhibit the cell growth in a dose-dependent manner as measured by MTT assay, which was associated with morphological change and apoptosis induction. Furthermore, WEEH treatment decreased the level of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthease (iNOS) expression without significant changes in the expression of COX-1, which was correlated with a decrease in prostaglandin E2 (PGE2) and NaNO2 synthesis. These findings suggest that WEEH may be a potential chemopreventive and chemotherapeutic agent for the control of human cancer cells. (Cancer Prev Res 13, 130-136, 2008)

Keywords: Euphorbiae humifusae, Hep3B, COX-2, iNOS

Share this article on :

Related articles in JCP

Most KeyWord