To confirm synergistic anticancer activity against mouse sarcoma, combination of water extract (HaWD80) of Albizzia julibrissin and doxorubicin was applied in vivo and in vitro. In sarcoma cells treated with HaWD80 or doxorubicin alone, IC₅₀ of HaWD80 and doxorubicin was 12.5Ռg/ml and 0.25Ռg/ ml, respectively. In sarcoma cells treated with mixture of HaWD80 and doxorubicin, however, IC₅₀ of HaBD80 and doxorubicin against the cells was 1.6Ռg/ml and 0.13Ռg/ml, respectively. Therefore mixture of HaWD80 and doxorubicin appeared synergistic anticancer activity against the sarcoma cells in vitro, because ԳFIC was 0.648Ռg/ml. Tumor Inhibition Ratio (TIR) of the mouse bearing the sarcoma treated doxorubicin (2.5 mg/kg) or HaWD80 (50 mg/kg) alone was 25.66% and 4.61%, respectively. However, TIR of the mouse bearing the sarcoma treated simultaneously doxorubicin (2.5 mg/kg) and HaWD80 (50 mg/kg) was 29.28%. Consequently mixture of HaWD80 and doxorubicin appeared synergistic anticancer activity against mouse sarcoma in vitro and in vivo. (Cancer Prev Res 17, 0-243, 2012)

Keywords: Albizzia julibrissin, Mouse sarcoma, Synergistic, Doxorubicin, Julibroside, In vivo