Reactive oxygen species (ROS) possess highly reactive response to macromolecules such as carbohydrate, nucleic acid, protein and amino acid in organism. Thus, various materials have been developed by researchers to remove ROS. So far, anti-oxidant material was purified from lots of plants and was applied for the production of health care products or skin care goods. In present research, an anti-yeast substance (AYS) purified from Rahnella aquatilis strain anti-yeast (AY) 2000 was verified for the ability of anti-oxidant and anti-histamine. H₂O₂ was scavenged by 21.2% and 20.3% with 0.02 and 0.04% AYS concentration, respectively. Especially, H₂O₂ was removed by 55.7% with 0.08% AYS concentration. In addition, AYS showed the effect of cell protection on HaCaT keratinocyte exposed to 0.25 mM H₂O₂. Furthermore, 0.005∼0.08% AYS had an excellent scavenging effect against OH radical in dose-dependent pattern. Moreover, we examined the effect of AYS on the compound 48/80-induced histamine release in human mast cell (HMC)-1. The culture supernatants were harvested, and under 1∼100 Ռg/ml concentrations, AYS inhibited histamine release in HMC-1 stimulated with compound 48/80 (300 Ռg/ml). These results indicated that AYS might be an useful skin care agent for anti-fungus as well as anti-oxidant and anti-histamine. (Cancer Prev Res 17, 0-231, 2012)

Keywords: ROS, Anti-yeast substance, Rahnella aquatilis, Anti-oxidant, Anti-histamine