Journal of Cancer Prevention 2014; 19(4): 247-252
Published online December 31, 2014
© Korean Society of Cancer Prevention
Eui-Yeun Yi1, Kyung-Suk Han2, and Yung-Jin Kim1
1Department of Molecular Biology, College of Natural Science, Pusan National University, Busan, Korea, 2Biochem c.a.l.s., Ltd., Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea
Correspondence to :
Yung-Jin Kim, Department of Molecular Biology, College of Natural Science, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 609-735, Korea, Tel: +82-51-510-2176, Fax: +82-51-513-9258, E-mail: firstname.lastname@example.org
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Angiogenesis is important processes for tumor growth and metastasis. Anti-angiogenesis target therapy has recently been known to be new anti-cancer therapeutic strategies. Natural products such as traditional medicine comprise a major source of angiogenesis inhibitors.
Keywords: Angiogenesis, Natural products,
Angiogenesis is a process of remodeling such as sprouting and growth of blood vessels.1 It is regulated under the control of balance between stimulators and inhibitors.2 In addition, angiogenesis involve complex and diverse cellular actions such as degradation of extracellular matrix, proliferation and migration of endothelial cells, and morphological differentiation of endothelial cells to form tubes.3,4
Many traditional oriental medicines consist of medicinal products from plants and animals that are used for treatments.5 It has been practiced in China, Korea, Japan, and other Asian countries for many centuries.6 In many cases, traditional medicinal products showed anti-tumor activities and they have been considered as candidates for novel cancer therapeutics.7,8
Although many studies on the effects of
HUVECs were purchased from InnoPharmaScreen Inc. (Asan, Korea). Basic fibroblast growth factor (bFGF) and heparin were obtained from PeproTech Inc. (Rocky Hill, NJ, USA). M199, fetal bovine serum (FBS), penicillin and streptomycin were purchased from WELGEN Inc. (Daegu, Korea). Matrigel was purchased from Collaborative Biomedical Products (Bedford, MA, USA) and used for the tube formation assay. Trans-well filter chambers (8-μm pores) were purchased from Corning-Costar (Cambridge, MA, USA).
2. Preparation of
3. Cell culture
HUVECs were grown in M199, supplemented with heat-inactivated 20% FBS (WELGEN Inc.), 20 ng/mL of bFGF, 100 units/mL of penicillin and 100 μg/mL of streptomycin in a 37°C incubator with a humidified atmosphere containing 5% CO2.
4. MTT assay for cell viability
The effect of extract of
5. In vitro tube formation assay
Before performing the test, 0.3 mL matrigel was transferred to 24-well plate and incubated for 30 minutes. HUVECs (2 × 104 cells) were plated on a layer of polymerized matrigel and treated with or without extract of
6. In vitro wounding migration assay
Invasion assay was performed using a trans-well chambers system (Corning Inc., Cambridge, MA, USA) with 8.0-μm pore polycarbonate filter inserts. The upper side of trans-well was coated with 10 μL of matrigel (0.5 mg/mL) at room temperature for 1 hour. Complete media was plated in the lower parts of the trans-well chamber filters, and HUVECs (2 × 104 cells) and extract of
8. Data analysis and statistics
Data are presented as means ± standard deviation. P < 0.05 was considered significant.
Angiogenesis is the developmental process of new capillaries by sprouting from pre-existing vasculature,16,17 and is required as a significant component of a wide variety of physiological process and pathological conditions.18 In 1971, Folkman15 was firstly built up that angiogenesis is essential process in tumor growth. Since then, angiogenesis have been recognized as important strategies for cancer therapy.19,20 Angiogenesis are initiated by the secreted growth factors, chemokines/cytokines and other mediators.3 Tumor initiation and progression are also closely linked to angiogenesis. Thus, angiogenic responses in tumor could be targets for development of anti-cancer therapeutic drugs.5
The studies for novel anti-cancer drugs from natural products have been continued through the research of scientists worldwide in looking for new bio-active compounds.20
In this study, we investigated whether extract of
Research of the functional ingredients from the traditional medicinal products is very important for therapy development. Chemical composition of
In summary, the major findings reported here are that extract of
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