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Journal of Cancer Prevention

Journal of Korean Association of Cancer prevention 1998; 3(2): 115-121

Published online June 30, 1998

© Korean Society of Cancer Prevention

A New Assay System for Analysing the Inhibitors of Tumor Invasion
and Metastasis Using MMP-9/SEAP Vector

Hee-Jae Cha, Joo-Won Jeong, You Mie Lee, Motoharu Seiki1,
Ok Hee Kim2 and Kyu-Won Kim


During the steps of tumor metastasis, matrix metalloproteinase-9 (MMP-9) is
one of the most important enzymes and acts a key role to degrade the extracellular
matrix. Therefore suppressing the expression of MMP-9 may inhibit the tumor
invasion and metastasis. In this study, we developed the assay system to analyze
the expression of MMP-9 by constructing the MMP-9 reporter vector and confirming
the action of reporter protein by SEAP assay. Furthermore, we confirmed the
expression pattern of MMP-9 is similar to that of Northern blot and gelatin
zymogram assay. This assay system enables to detect the expression of MMP-9
by treating only simple methods. Therefore, we can apply this system to screen
the MMP-9 inhibitors in various candidate compounds and factors.

Keywords: MMP-9, SEAP vector, Invasion, Metastasis

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