The aim of this study was to examine the reactive oxygen species (ROS) scavenging effects of Jeju waters containing vanadium components; S1 (8.0±0.9Ռg/l), S2 (24.0±2.0Ռg/l), and S3 (26.0±2.0Ռg/l). Human Chang liver cells were incubated for 10 passages in media containing deionized distilled water (DDW group) and Jeju waters (S1, S2, and S3 groups). DDW and Jeju waters did not show cytotoxicity and scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical. Electron spin resonance spectrometer data showed that cells exposed to Jeju waters showed significantly scavenged superoxide anion induced by the xanthine/xanthine oxidase system and hydroxyl radicals induced by Fenton reaction (H₂O_2＋FeSO₄) compared to cells exposed to DDW. Furthermore, the S3 group significantly scavenged intracellular ROS compared to the DDW group, as measured by spectrofluorometry, flow cytometry, and confocal microscopy after staining with 2',7'-dichlorodihydrofluorescein diacetate. These results suggest that vanadium components-containing Jeju waters exhibited antioxidant effect by scavenging ROS. (Cancer Prev Res 15, 111-117, 2010)

Keywords: Reactive oxygen species, Vanadium, Antioxidant effect