According to GLOBOCAN estimates of cancer incidence and mortality for 2012 in 184 countries worldwide, ovarian cancer is the seventh most common malignancy (239,000 cases) and the eighth leading cause of cancer mortality (152,000 deaths) in women.1 In the United States, 22,440 new cases of ovarian cancer and 14,080 ovarian cancer deaths were estimated in 2017.2 Risk factors for ovarian cancer include age, family history, hormone replacement therapy, obesity, nulliparity, and smoking.3 In contrast, factors associated with the decreased lifetime number of ovulatory cycles, such as pregnancy, breast feeding, use of oral contraceptives, fallopian tube ligation, and hysterectomy, have been reported to reduce ovarian cancer risk.3 Ovarian cancer has been associated with poor prognosis and the highest mortality rate of all gynecological malignancies due to the presence of advanced stages of disease at the time of diagnosis. About 70% of the patients are diagnosed with distant-stage disease, for which 5-year survival rate is 25% to 30% whereas the survival rate is more than 90% for patients diagnosed with localized disease.4,5 The lack of specific symptoms and reliable molecular biomarkers makes early detection of ovarian cancer challenging.6–8 Furthermore, due to the heterogeneous nature of the tumors and resistance to conventional chemotherapeutic drugs, the efficacy of current treatments for ovarian cancer has been limited. Therefore, there is an urgent need for the development of novel and effective approaches to reduce the incidence and mortality of the disease. It is of particular interest to note that chemoprevention provides a promising strategy for ovarian cancer risk reduction.

Epidemiological studies have documented that there is a wide variability in the incidence of ovarian cancer among different countries, with the highest rates in Western countries and the lowest rates in Asian and African countries, suggesting that dietary factors and lifestyle may play a key role in the etiology of the disease.9,10 Increasing evidence has demonstrated that high dietary intake of lycopene, one of the bioactive components in a variety of foods, is associated with reduced risk of ovarian cancer, supporting the protective role of lycopene against ovarian cancer.11–16 Lycopene, which is a lipophilic red pigment, is the most abundant carotenoid in tomatoes and is also found, to a lesser extent, in watermelons, pink grapefruits, carrots, apricots, and guavas.17 Lycopene is an acyclic isomer of β-carotene and it lacks vitamin A activity.18 Due to its highly unsaturated hydrocarbon chain with 11 conjugated and two unconjugated double bonds, lycopene exerts effective singlet oxygen quenching and peroxyl radical scavenging activities, resulting in strong antioxidant capacity.19 Although the chemopreventive effects of lycopene have been primarily attributed to its antioxidant properties, other potential mechanisms of action have also been reported in cancer prevention. In vitro and in vivo studies have shown that lycopene modulates various cellular processes, including growth factor signaling pathways (insulin-like growth factor, platelet-derived growth factor, and vascular endothelial growth factor [VEGF]),20–22 cell cycle,23,24 apoptosis,25,26 and metastasis,27,28 by acting on molecular targets, such as phosphoinositide 3-kinase, AKT, mitogen-activated protein kinases, NF-κB, activating protein-1 [AP-1], TNF-α, COX-2, interleukin-6, matrix metalloproteinases, and β-catenin. Moreover, lycopene has been reported to counteract the suppression of gap junctional communication during carcinogenesis, leading to inhibition of cell growth.29 Efficacy studies have demonstrated that lycopene in combination with other natural anticancer compounds has synergistic inhibitory effects on tumor growth.30–33 In addition, lycopene has been shown to act as a chemosensitizer34–36 and a radiosensitizer37,38 for cancer cells, and ameliorate the adverse side effects of the anticancer therapies.35,37

Although epidemiological studies have shown the inverse association between dietary lycopene intake and ovarian cancer risk, the molecular mechanisms underlying the preventive effects of lycopene on ovarian carcinogenesis have not been fully elucidated. Among the animal models of ovarian cancer, the laying hen (Gallus domesticus) has great potential to provide the most relevant preclinical model that recapitulates the etiology and progression of the disease in human. The fact that laying hen is the only non-human animal that spontaneously develops ovarian cancer at a high incidence makes the laying hen model unique among animal models to study the ovarian tumorigenesis.39 Importantly, similar to humans, laying hens have been shown to have high ovulatory rates, suggesting the possibility of a similar pathogenesis associated with ovulation-induced inflammatory event, resulting in DNA damage to ovarian epithelial cells in both hen and human ovarian cancers.40 As in women, use of oral contraceptives to inhibit ovulation in the laying hens has been reported to be associated with a risk reduction of ovarian cancer.41 In addition, ovarian tumor cells in laying hens exhibit molecular characteristics similar to those of human ovarian cancer cells, including the expression of several biomarkers (cytokeratin, cancer antigen 125 [CA125], VEGF, epidermal growth factor receptor [EGFR], human epidermal growth factor receptor 2, CYP1B1, and proliferating cell nuclear antigen [PCNA]).42–45 Interestingly, the histopathological subtypes of ovarian tumors in humans (serous, endometrioid, mucinous, and clear cell carcinomas) are also observed in hens.46 Therefore, in the present study, we utilized the laying hen model to investigate the effects of lycopene on the development of spontaneous ovarian cancer, providing mechanistic insights into the mode of action of lycopene in the prevention of ovarian cancer.

1. Animal husbandry and experimental design

A total of 150 brown laying hens (ATAK-S hybrid, Gallus domesticus) at 102 weeks of age were maintained in accordance with the animal welfare regulations at the Ankara Poultry Research Station, Turkey. Hens were housed five per cage under a 14-hour light and 10-hour dark cycle with access to water and diet ad libitum. Animals were assigned randomly to three groups with 50 hens in each group: a control group (0 mg of lycopene per kg of diet) and two treatment groups (200 mg or 400 mg of lycopene per kg of diet, equivalent of approximately 26 and 52 mg/d/hen, respectively). Ingredients and composition of basal diet are shown in Table 1. Experimental diets were prepared by supplementing the basal diet with water-soluble lycopene (Redivivo, CWS/S-TG), which was supplied by DSM (Istanbul, Turkey). Diets were stored in black plastic containers at 4°C to avoid photooxidation.

2. Necropsy and tissue collection

At the end of lycopene administration for 12 months, blood samples were collected from the axillary vein, and animals were euthanized, followed by necropsy. Hens were evaluated for abnormal ovarian morphology (tumors, cysts, and solid mass) by gross examination. Tumor incidence and the number and size of tumors were recorded.

3. Histological analysis

Upon necropsy, ovarian tissues were removed and placed in 10% neutral-buffered formalin. After fixation in formalin, tissues were processed by paraffin embedding, sectioned at 6 μm, stained with hematoxylin and eosin, and examined under light microscope. Histological types were classified as normal, tumor, adenocarcinoma, or lesions.

4. Analysis of serum levels of malondialdehyde and lycopene

Serum was separated by centrifuging the blood sample at 3,000 × g for 10 minutes and analyzed for malondialdehyde (MDA) and lycopene levels by high-performance liquid chromatography (HPLC) using a Shimadzu HPLC system with an SPD-10A VP UV-Vis detector and an ODS C18 column (250 × 4.6 mm internal diameter and 5 μm particle size) (Shimadzu, Tokyo, Japan) as described previously.47

To quantify MDA concentration, 300 μL of serum sample was homogenized in a mixture of 200 μL of HClO₄ (0.5 M) and 100 μL of 500 ppm butylated hydoxytoluene (BHT), and centrifuged. An aliquot of 20 μL of supernatant was injected into an HPLC column. Samples were eluted with a mobile phase containing 30 mM of KH₂PO₄-methanol (82.5:17.5, v/v, pH 3.6) at a flow rate of 1.2 mL/minute. Chromatograms were acquired at 250 nm.

To measure the lycopene concentration, serum sample was mixed with a KOH/ethanol solution (1:5) containing 0.1% BHT, followed by addition of deionized water. Serum lycopene was extracted three times with addition of hexane. Hexane extracts were dried under nitrogen in dark and subsequently dissolved in 100 μL of mobile phase. All extracts were kept on ice and protected from light. HPLC analysis was performed on a column with methanol : acetonitrile : dichloromethane : water (7 : 7 : 20 : 0.16) containing 20 mmol/L ammonium acetate at flow rate of 1 mL/min and detection was performed at 470 nm.

5. Western blot analysis

Protein lysates from ovarian tissues (normal or tumor tissues) were prepared as described previously.48 Briefly, ovarian tissues were homogenized in lysis buffer (10 mM Tris-HCl [pH 7.4], 0.1 mM NaCl, 0.1 mM phenylmethylsulfonyl fluoride and 5 μM soluble soybean powder [Sigma, St. Louis, MO, USA] as trypsin inhibitor) at 1:10 (w/v), and centrifuged at 15,000 ×g at 4°C for 30 minutes. Supernatant was collected and protein concentration was determined. Protein sample was mixed with Laemmli sample buffer containing β-mercaptoethanol, and incubated at 95°C for 5 minutes. Twenty μg of total protein was separated on 10% SDS PAFE and transferred onto nitrocellulose membrane (Schleicher and Schuell Inc., Keene, NH, USA). Membranes were washed twice for 5 minutes with PBS and incubated in blocking solution (PBS containing 1% bovine serum albumin) for 1 hour. Blots were then incubated overnight at 4°C with primary antibodies against NF-κB, STAT3, nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2), protein inhibitor of activated STAT 3 (PIAS3), and heme oxygenase 1 (HO-1) (Abcam, Cambridge, UK) diluted at 1:1,000 in the PBS buffer containing 0.05% Tween 20. Blots were washed and incubated with horseradish peroxidase- conjugated goat anti-mouse immunoglobulin G (Abcam). Detection was performed using diaminobenzidine and hydrogen peroxide as substrates. β-actin antibody (Sigma) was used as normalization control. Quadruplicates were run for each sample. Protein intensities were quantitated densitometrically using ImageJ analysis system (National Institute of Health, Bethesda, MD, USA).

6. Statistical analysis

Differences in the tumor incidences and numbers in the groups were evaluated statistically using the chi-square χ² test. Data were analyzed via analysis of variance using the general linear model with the SAS program (2002) (SAS Institute, Cary, NC, USA) to determine the effects of lycopene supplementation on tumor diameter, protein staining intensities, and serum metabolites. When a significant F statistic (P ≤ 0.05) in the ANOVA was noted, the mean procedure was performed to separate means that were significantly different (P < 0.05).

1. Lycopene reduces the incidence and the size of spontaneous ovarian tumors in laying hens

In this pilot study, we conducted a three-armed controlled trial to prospectively test the effects of lycopene intervention on the development of spontaneous ovarian cancer in the laying hen model. A total of 150 laying hens at 102 weeks of age were randomly assigned into groups of 50: a control group (0 mg of lycopene per kg of diet) and two treatment groups (200 mg or 400 mg of lycopene per kg of diet). At the end of 12-month period of lycopene administration, the necropsy examination of the hens indicated a preventive effect of lycopene on the incidence of spontaneous ovarian cancer (Table 2). Our finding that 40% of the hens (20/50) fed the control diet developed ovarian cancer spontaneously was consistent with previously published data.49 As shown in Table 2, lycopene supplementation significantly decreased the tumor incidence in the ovary of laying hens in comparison with control animals (P < 0.010). Gross pathology results showed that 34% (of the hens 17/50) in 200 mg/kg group had ovarian tumors whereas only 14% of the (7/50) animals in 400 mg/kg group developed ovarian cancer, indicating that lycopene acts in a dose-dependent manner in ovarian cells. Moreover, lycopene treatment significantly reduced both the number and size of ovarian tumors compared to the control group (Table 2). While 8% of the control animals had multiple ovarian tumors, none of the hens in the treatment groups developed multiple tumors. Hens in the control group had an average of 0.48 tumors whereas the animals fed 200 mg/kg and 400 mg/kg of lycopene had 0.34 and 0.14 tumors, respectively (P < 0.004). We observed that the mean tumor size was 6.64 mm in the control group while it was 3.98 mm and 1.56 mm in 200 mg/kg and 400 mg/kg groups, respectively (P < 0.005), indicating a significant inhibition of tumor growth and further supporting the preventive role of lycopene in ovarian cancer.

2. Lycopene reduces adenocarcinoma of the ovary in laying hen

At the time of necropsy, animals were examined extensively for the presence of ovarian abnormalities. Gross examination demonstrated that hens with normal ovaries had a higher number of developing follicles compared to those with ovarian abnormalities. Non-tumor ovarian abnormalities in hens were characterized by the presence of atretic follicles with fat globules in the degenerating granulosa and theca cells as well as cysts. Macroscopic assessment also showed that ovarian abnormalities in laying hens were associated with atresia of 2–3 large preovulatory hierarchical follicles and the presence of multiple cysts. In hens with cancerous ovary, the tumors had solid nodular masses and no large yolky preovulatory follicles were observed in the ovary (Fig. 1). In some hens, tumors were metastasized to peritoneal organs with profuse ascites. Representative images of histologic analysis of ovarian tissues from normal and carcinoma cases are shown in Figure 2. Features of endometrioid type carcinoma were observed in some hens as glandular structures lined by columnar, cuboidal, or oval epithelial cells. Poorly differentiated or mixed ovarian carcinomas were also identified in some cases.

Histopathological analysis of ovarian tissues demonstrated a significant difference in the rate of spontaneous adenocarcinoma between control and treatment groups (Table 2). While adenocarcinoma was observed in 20/50 of the hens (40%) in the control group, the animals fed lycopene developed significantly less adenocarcinoma with 17/50 (34%) and 7/50 (14%) in 200 mg/kg and 400 mg/kg groups, respectively (P < 0.03). In the control group, 14/50 of the animals (28%) had serous carcinomas and 6/50 (12%) had mucinous carcinomas. In 200 mg/kg lycopene group, there were 12/50 birds (24%) with serous and 5/50 (10%) mucinous carcinomas, and in 400 mg/kg lycopene group, there were 4/50 birds (8%) with serous and 3/50 (6%) mucinous carcinomas.

3. Lycopene decreases serum levels of an oxidative stress marker in laying hens

As shown in Table 3, no lycopene was detected in the serum samples of hens from control group. However, hens fed lycopene had significantly elevated serum lycopene levels in comparison with the control animals (P < 0.001). Additionally, the increase in the level of serum lycopene was shown to be dose-dependent (6.29 nmol/mL and 13.75 nmol/mL in 200 mg/kg and 400 mg/kg groups, respectively). To explore the effects of lycopene supplementation on oxidative stress in the laying hen model, we analyzed the levels of serum MDA, a well-documented biomarker of oxidative stress, using HPLC (Table 3). Our data showed that hens fed lycopene exhibited the significantly decreased serum MDA levels in comparison with the control hens, and the reduction was in a dose-dependent manner (P < 0.001). Mean serum MDA level was determined as 2.26 nmol/mL in the control group while it was found to be 1.65 nmol/mL and 0.90 nmol/mL in 200 mg/kg and 400 mg/kg groups, respectively. These results demonstrate an inverse correlation between levels of lycopene and oxidative stress, supporting the hypothesis that lycopene supplementation could ameliorate oxidative stress in the hen ovary.

4. Lycopene suppresses the NF-κB signaling

It is well established that oxidative stress, defined as an imbalance between production and elimination of reactive oxygen species (ROS), can cause chronic inflammation, leading to transformation of a normal cell to a tumor cell. The key mediators in the inflammatory pathways modulate expression and/or activity of various transcription factors, including NF-κB, STAT3, and Nrf2. Therefore, to elucidate the molecular mechanisms underlying the lycopene-induced inhibition of the pathogenesis of ovarian cancer, we first examined the effect of lycopene on the NF-κB signaling pathway. Representative Western blots are shown in Figure 3A. Quantitative analysis of protein samples extracted from the ovarian tumors in the control and lycopene- fed hens showed that lycopene significantly reduced the expression level of NF-κB, suggesting that NF-κB signaling may be a prime target for the anti-tumor effects of lycopene on ovarian cancer. As illustrated in Figure 3B, the inhibitory effect of lycopene on NF-κB is dose-dependent; there is a reduction of 38% and 64% in NF-κB levels in hens treated with 200 mg/kg and 400 mg/kg of lycopene, respectively (P < 0.001).

5. Lycopene decreases the expression of STAT3 by inducing the expression of protein inhibitor of activated STAT3 in the ovary of laying hen

To further characterize the molecular mechanisms involved in the lycopene-induced changes in the ovarian carcinogenesis, we determined the effects of lycopene on the expression of STAT3, another key pro-inflammatory transcription factor activated in response to a variety of growth factors and cytokines.50 Mutations resulting in constitutively activated STAT3 have been reported to promote tumorigenesis through deregulation of cell cycle and apoptosis in ovarian cells.51 Our results demonstrated that lycopene significantly downregulates the expression of STAT3 in the ovary (Fig. 3C). We observed a reduction of 48% and 68% (P < 0.05) at protein levels of STAT3 in hens fed 200 mg/kg and 400 mg/kg of lycopene, respectively. In addition, we analyzed the expression levels of the protein inhibitor of activated STAT3 (PIAS3), an upstream modulator of STAT3 that attenuates the transcriptional activity of STAT3 by inhibiting its DNA binding.52 Western blot analysis showed that lycopene significantly and dose-dependently upregulated the expression of PIAS3 in ovarian cells of laying hens (Fig. 3D). Taken together, these results suggest that overexpression of PIAS3 by lycopene contributes to suppression of tumor growth by inhibiting the function of constitutively active STAT3 in ovarian cancer cells.

6. Lycopene enhances the expression of nuclear factor erythroid 2-related factor 2 and heme oxygenase 1 in the ovary of laying hen

A major mechanism in the cellular defense against oxidative stress is the activation of the transcription factor Nrf2, the master regulator of antioxidant and anti-inflammatory responses.53 Therefore, enhancing the Nrf2 activity is a key approach to prevention of cancer and other diseases in which oxidative stress contributes to the pathogenesis. To investigate whether lycopene exerts antioxidant effects on ovarian cancer, we assayed the expression levels of Nrf2 and its downstream target HO-1 enzyme in tumors from control and treatment groups by Western blotting. As illustrated in Figure 3E and 3F, lycopene intervention significantly and dose-dependently increased the expression levels of Nrf2 and HO-1 in hen ovarian tumors. We observed an increase of 44% and 74% in Nrf2 levels (P < 0.01 and P < 0.001), and an increase of 30% and 53% in HO-1 levels (P < 0.05 and P < 0.001) in hens fed 200 mg/kg and 400 mg/kg of lycopene, respectively. Our findings provide the molecular basis of antioxidant activity of lycopene in the context of its chemoprevention of ovarian cancer.

In recent years, there has been considerable interest in the potential role of lycopene in the chemoprevention of ovarian cancer. However, only a limited number of studies have examined the relationship between dietary intake of lycopene and ovarian cancer risk, and the results have been inconsistent. A population-based study conducted by Cramer et al.15 showed a significant inverse association between tomato sauce intake and ovarian cancer risk in premenopausal women.54 Kiani et al.13 also reported that fruit- and vegetable-rich diet and especially tomatoes were associated with a reduced ovarian cancer risk. In a case-control study, Tang et al.11 demonstrated that with the exception of lycopene, significant reductions in risk of ovarian cancer were associated with the intake of a variety of fruits and vegetables. But the amount of lycopene intake was very low in this study compared with those in other studies. Despite these favorable results, other epidemiological studies reported that intake of lycopene or other carotenoids showed no association with ovarian cancer risk.16,55–59 In addition, a pooled analysis of 10 cohort studies demonstrated that intake of major carotenoids was not associated with the risk of ovarian cancer.12 Helzlsouer et al.60 reported no association between serum lycopene levels and ovarian cancer risk whereas Jeong et al.61 observed a significant inverse association between plasma levels of lycopene and ovarian cancer risk.

To the best of our knowledge, the present study is the first investigation of the preventive effects of lycopene on spontaneous ovarian cancer formation using the laying hen model. Our findings demonstrated that lycopene reduced the incidence and the size of ovarian tumors in laying hens, possibly due to its antioxidant and anti-inflammatory activities. Previous studies have shown that the laying hen provides a biologically relevant preclinical model to characterize the molecular mechanisms underlying pathogenesis of human ovarian cancer as well as to test the effects of agents on prevention of the disease. In 1987, Fredrickson49 reported that 32% and 8% of white leghorn hens developed spontaneous ovarian and oviductal adenocarcinomas, respectively. In addition, granulosa and sertoli cells tumors were observed in 8% of the hens. As in women, high ovulation rate has been correlated with the risk of ovarian cancer in laying hens. Moreover, there is an age-related increase in the incidence of ovarian cancer in hens as egg production decreases. Molecular characterization of chicken ovarian cancer cells has shown high expression of several biomarkers, including EGFR, tumor-associated glycoprotein 72, PCNA, TGF-α, and CA125, as in human ovarian tumors.42,43 In addition, Barua et al.46 demonstrated that similar to humans, four histological subtypes, including serous, endometrioid, mucinous, clear cell carcinomas, are observed in chicken ovarian tumors.

It is well documented that ovarian carcinogenesis has been associated with chronic inflammation which is induced by oxidative stress.62 In eukaryotic cells, ROS are products of normal metabolic reactions and are required for stimulation of signal transduction pathways in response to cellular changes.63 However, when the equilibrium between ROS and endogenous antioxidants is perturbed, oxidative stress is generated, damaging important biomolecules, including DNA, RNA, lipids, and proteins, leading to neoplastic transformation.64 Oxidative stress modulates the expression of a variety of transcription factors, including NF-κB, STAT3, hypoxia-inducible factor 1-α, AP-1, p53, PPAR-γ, β-catenin/Wnt, and Nrf2, which mediate immediate cellular stress responses. Therefore, targeting ROS-induced signaling pathways by antioxidant agents offers an effective strategy for cancer prevention.

In the ovarian surface epithelium, the injury and tissue remodeling caused by ovulation trigger the inflammatory cascade. It has been reported that the inflammatory events caused by repetitive ovulation over the lifetime increase the risk of DNA damage during the repair process, predisposing the ovarian surface epithelial cells to neoplastic transformation by the accumulation of genetic mutations.65 The evidence that oral contraceptive use, parity and breast-feeding reduce ovarian cancer risk supports the role of inflammation in ovarian carcinogenesis.66

Lycopene is the most effective singlet oxygen quencher among 600 naturally occurring carotenoids, supporting its strong antioxidant activity.67 Several studies demonstrated that lycopene modulates ROS levels, leading to inhibition of ROS-induced cell damage.68 Excessive ROS has been shown to induce lipid peroxidation of polyunsaturated fatty acids.69 MDA, which is one of the end products of lipid peroxidation, is a commonly used biomarker for oxidative stress.70 We have previously reported that lycopene supplementation reduced the incidence of spontaneous leiomyomas of the oviduct in Japanese quail.71 Additionally, serum MDA levels were significantly reduced in lycopene fed birds, consistent with data previously published by others.72 Our present study showed that lycopene intervention resulted in a significant and dose-dependent reduction of serum MDA levels in laying hens, suggesting that lycopene exerts its chemopreventive effects via oxidative stress-induced signaling pathways. Based on this finding, we hypothesized that lycopene could ameliorate oxidative stress-induced inflammation in the ovary of laying hens through regulation of NF-κB, STAT3, and Nrf2 pathways.

NF-κB is a key transcription factor that acts as a molecular link between inflammation and cancer. The NF-κB pathway is activated by ROS in response to inflammatory cytokines and proteins, and plays a critical role in cell proliferation, survival, adhesion, differentiation and cell growth, inducing tumor initiation and progression.73,74 Our data showed that lycopene supplementation significantly reduced the expression of NF-κB in the ovary of laying hen, supporting the hypothesis that lycopene mediates its chemopreventive effects on the ovarian car-cinogenesis through inhibition of NF-κB signaling.

Molecular studies have shown that the JAK2/STAT3 pathway plays a regulatory role in oxidative stress as well as inflammation.75 JAK2/STAT3 signaling modulates cellular activities, including proliferation, migration, differentiation and death.76 In normal cells, the JAK2/STAT3 pathway is transiently activated primarily by growth factors and cytokines as well as toll-like receptors, microRNAs, and G protein-coupled receptors.77–80 However, in cancer cells, including breast, ovarian and prostate tumors, the JAK2/STAT3 pathway has been shown to be constitutively active.51 Therefore, it is essential to develop effective approaches to inhibit the JAK2/STAT3 signaling pathway for prevention and treatment of cancer. Our findings showed that lycopene significantly reduced the STAT3 expression while enhancing the expression of its upstream regulator PIAS3, suggesting that inhibition of STAT3 via overexpression of PIAS3 results in a protective effect against ovarian carcinogenesis.

The Nrf2 pathway plays a crucial role in cellular defense by sensing the level of oxidative stress induced by ROS, and regulating the expression of antioxidant proteins and detoxification enzymes, maintaining cellular redox homeostasis.81 In addition to its primary role in cytoprotection, the transcription factor Nrf2 is also involved in diverse cellular processes, including differentiation, proliferation, apoptosis, inflammation, and lipid synthesis.82 In the present study, we demonstrated that lycopene intervention significantly increased the expression levels of Nrf2 and its downstream target HO-1 in hen ovarian cancer cells, providing further support for the involvement of antioxidant activity of lycopene in chemoprevention of ovarian cancer.

In conclusion, our findings indicate that dietary intake of lycopene reduces the incidence and the size of spontaneous ovarian tumors in the laying hen model. We have also observed that lycopene exerts potent antioxidant and anti-inflammatory activities in ovarian cells by modulating diverse signaling pathways, including NF-κB, STAT3, and Nrf2, contributing to prevention of ovarian cancer. Taken together, these data provide further support and mechanistic insights into the chemo-preventive effects of lycopene on ovarian cancer in a biologically relevant in vivo model. Our results warrant further pre-clinical and clinical studies to assess the preventive effects of lycopene, and potentially improve the clinical outcomes and overall survival rates of patients with ovarian cancer.

No potential conflicts of interest were disclosed.