Fig. 3. The levels of N1-acetylspermidine is elevated in MCF-7 breast cancer cells treated with doxorubicin (DOX). Intracellular metabolite profiling of MCF-7 cancer cells treated with DOX is assisted by vertical tube gel electrophoresis and liquid chromatography-high resolution mass spectrometry (LC-HRMS). MS and MS/MS derived product ion spectra of N1-acetylspermidine were collected in the positive electrospray ionization (ESI) mode.

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