Fig. 4. Treatment with RPL9 siRNA suppresses the abilities of invasion, migration and sphere formation in CR-CSCs. (A, B) Representative light microscopy images and relative percentage of invasive (A) and migratory (B) CD133⁺ HT29 CSCs treated with NC siRNA or RPL9 siRNA. The invaded cells were marked with red arrows in (A). Scale bar, 100 μm. (C) Representative light microscopy images and relative efficiency of sphere formation in CD133⁺ HT29 CSCs treated with NC siRNA or RPL9 siRNA. Scale bar, 100 μm. The difference in sphere forming capacity was compared by calculating size and quantifying number of spheres. *P < 0.05; **P < 0.01 vs. NC siRNA treatment. RPL9, ribosomal protein L9; CR-CSC, colorectal cancer stem cell; NC, negative control.

© J Cancer Prev