Fig. 2. Use of CD133 CSC marker effectively isolates CR-CSCs. (A) Separation of HT29 CSC population from cancer cell population using CD133 antibody. (B) Relative levels of CD133 mRNA and CD133 protein in CD133^- and CD133⁺ fractions. **P < 0.01 vs. NC siRNA treatment. CR-CSC, colorectal cancer stem cell; NC, negative control.

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