Journal of Cancer Prevention

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Fig. 2.

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Fig. 2. Effects of Dendropanax morbifera Leveille extract (DME) on cell migration and invasion.
(A, B) Once cells were confluent on six-well plates, they were wounded with a scratch and treated with various concentrations of DME from 0 to 40 µg/mL. The migration area was measured after 24 hours. Magnification, ×100. (C, D) Cells were added to the upper chamber with 1.5 mL of serum-free Dulbecco’s modified Eagle’s medium (DMEM), and DMEM with 10% FBS was added to the lower chamber. After incubation for 24 hours, non-invading cells in the upper chamber were removed using a Q-tip, and the cells invading the lower chamber were fixed with 4% paraformaldehyde and stained with 0.5% crystal violet. Invasive cells were then examined under a microscope and quantified using ImageJ (National Institutes of Health). Magnification, ×100. The results are presented as the mean ± SD. Statistical differences were determined using Student’s t-tests (*P < 0.05, **P < 0.01, ***P < 0.001 vs. control).
J Cancer Prev 2023;28:185~193
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