Fig. 3. Apoptosis induction of liposomal treatment.
(A) Live, dead and apoptotic cell distribution of PC3 cells were quantitatively assessed by annexin V/propidium iodide staining using image-based cytometer. (B) Each column represents the mean ± regulation of apoptotic genes. All data were performed in triplicate and values are means of the two different experiments ± standard deviation. Apoptotic cells detected by Hoechst staining. (C) RT-qPCR mRNA expressions of BAX, APAF, Caspase 3, Caspase 8 were analyzed 72 hours after treatment. (D) Treatment cells with liposomal Quer significantly inhibited the expression levels of antiapoptotic Bcl-2 protein, and (E) upregulated apoptotic cytochrome c protein. UT, untreated; LP, nanoliposomes; LP-HA, hyaluronic acid-modified nanoliposomes; Quer, quercetin; LP-Quer, quercetin-loaded nanoliposomes; LP-Quer-HA, hyaluronic acid-modified, quercetin-loaded nanoliposomes. *P < 0.001 vs. UT, ^#P < 0.001 vs. LP-Quer-HA, ^‡P < 0.001 vs. Quer, **P < 0.001 vs. all groups.

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