Fig. 2. Baicalein attenuates α-MSH-stimulated tyrosinase activity and tyrosinase-related protein expression. (A) The 1 µM α-MSH was treated to the cells for 12 hours, then baicalein or arbutin was added. Spectrophotometric analysis measuring absorbance at 570 nm was conducted to determine tyrosinase activity. *P < 0.05 and ^#P < 0.05 compared to the control and α-MSH-treated cells. The Western blot analysis was performed by using (B) tyrosinase antibody and (C) TRP-2 antibody, with actin used as the loading control. α-MSH, α-melanocyte-stimulating hormone; TRP, tyrosine-related protein.

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