Fig. 1. Baicalein inhibits UVB- and α-MSH induced enhancement of melanin contents in B16F10 cells. (A) The cytotoxic effect of baicalein at concentrations of 0, 1, 5, 10, or 20 µM was determined by the MTT assay. (B) UVB radiation was used to determine the amount of melanin. Cells were pretreated with baicalein for 1 hour, then UVB was irradiated. Spectrophotometric analysis at 405 nm absorbance determined the melanin concentration. *P < 0.05 and ^#P < 0.05 compared to the control and UVB-exposed cells, respectively. (C) After pre-treatment of cells with 1 µM α-MSH for 12 hours, baicalein was applied to the cells. The melanin content was analyzed spectrophotometrically by measung absorbance at 405 nm. α-MSH, α-melanocyte-stimulating hormone; MTT, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] bromide. *P < 0.05 and ^#P < 0.05 compared to the control and α-MSH-treated cells.

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