Journal of Cancer Prevention

eISSN 2288-3657
pISSN 2288-3649
Fig. 3.

Download original image

Fig. 3. Effects of BTE on iAs-induced EMT. After 330 days of treatment, the results show downregulation of epithelial markers (E-cadherin and desmoplakin) and upregulation of mesenchymal markers (vimentin, N-cadherin, Snail, Slug, Twist, and Zeb1) in the iAs treated mice, at the protein and mRNA levels; while in the iAs + BTE treated mice, these results were reversed. (A) displays representative Immunoblot bands of the respective EMT markers. The lanes 1, 2, and 3 represent control, iAs, and iAs + BTE treated mice respectively. β-Actin is the loading control. (B) displays representative RT-PCR bands of the respective EMT markers. (C) displays a bar graph representing fold change in band intensities of the immunoblots of respective EMT markers. The results are expressed as mean of three independent experiments ± standard deviation. EMT, epithelial to mesenchymal transition; RT-PCR, reverse transcriptase PCR; iAs, inorganic arsenic; BTE, black tea extract. The modulation of the EMT markers in the iAs treated mice tissues, with respect to the control mice, is highly significant at aP < 0.0001. The alteration of EMT markers in the iAs + BTE treated mice, with respect to the iAs treated mice, is significant at bP < 0.001 and highly significant at cP < 0.0001. (D) shows a bar graph representing the fold change in RT-PCR band intensities of the respective EMT markers. The alteration in PCR bands of respective EMT markers in the iAs treated mice, with respect to the control group, is highly significant at aP < 0.0001. The modulation of bands in the iAs + BTE treated mice, with respect to the iAs treated mice, is significant at bP < 0.001 and highly significant at cP < 0.0001.
J Cancer Prev 2023;28:12~23 https://doi.org/10.15430/JCP.2023.28.1.12
© J Cancer Prev
© Korean Society of Cancer Prevention. / Powered by INFOrang Co., Ltd