Fig. 4. Effects of exhaustive exercise on Nrf2-driven HO-1 expression during exhaustive exercise and the 1-hour recovery period as well as resting conditions in human PBMCs. (A) HO-1 expression levels in human PBMCs. Protein levels were measured using Western blot analysis with a rabbit polyclonal HO-1 antibody. (B) Nrf2-ARE binding activity in human peripheral blood mononuclear cells. Nuclear proteins (10 μg) were resolved by electrophoretic mobility shift assay. Results are means ± standard deviation; n = 16 per group. Nrf-2, NF-E2 related factor 2; PBMC, peripheral blood mononuclear cell; HO-1, heme oxygenase-1; ARE, antioxidant response elements; IAE, immediately after exercise; 1h-RAE, 1-hour recovery after exercise. ***P < 0.001.

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