Journal of Cancer Prevention

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Fig. 2.

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Fig. 2. Comparison of the inflammatory signaling pathway during exhaustive exercise and 1-hour recovery in human PBMCs. (A) Protein extracts (40 μg) were loaded onto a 10% SDS PAGE, electrophoresed, and subsequently transferred onto a polyvinylidene difluoride membrane. Western blot analysis was probed with a rabbit polyclonal COX-2 antibody. (B) The nuclear extract (10 μg) was incubated with radiolabeled oligonucleotides containing the NF-κB consensus sequence for analysis by electrophoretic mobility shift assay. (C) Cytosolic p-IκBα levels in human PBMCs. Results are means ± standard deviation; n = 16 per group. PBMC, peripheral blood mononuclear cell; IAE, immediately after exercise; 1h-RAE, 1-hour recovery after exercise; p, phospho. *P < 0.05, **P < 0.01, ***P < 0.001.
J Cancer Prev 2023;28:3~11
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