Fig. 3. Sangju honey decreases migration abilities of oral cancer cells. (A, B) The migration rate of Ca9-22 cells was measured every 3 hours after treatment with 0.25% or 0.5% of honey. (C, D) The migration rate of YD-10B cells was determined every 3 hours after treatment with 0.25% or 0.5% of honey. Cell migration was observed with a light microscope at 5 × magnification. (E) Western blot analysis on the expression of E-cadherin and N-cadherin in Ca9-22 and YD-10B cells after treated with 0.25% or 0.5% of honey for 48 hours. (F) Relative protein expression levels in Ca9-22 and YD-10B cells were quantified by ImageJ. The experiments were conducted in triplicate and shown as the mean ± standard deviation (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 compared to control.

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