Journal of Cancer Prevention

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Fig. 4.

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Fig. 4. Cedrol suppresses the tumor growth and the expression of MCM7 in colorectal tumor xenograft mice. BALB/c nude mice (n = 5/group) were inoculated subcutaneously with HT29 cells. After the tumor formation, the mice were treated intravenously with vehicle (●), 3 mg/kg of ADR as a positive control (■), or 50 mg/kg of cedrol (▲). (A) The tumor volume was measured and calculated as described in Materials and Methods. (B, C) The tumor tissues were removed at 14 days after the first injection and weighed. (D) Mouse body weight was measured. Data are presented as mean ± standard deviation. *P < 0.05 and **P < 0.01 vs. control group. (E) Histological analysis. Tumor tissues were fixed with 10% formaldehyde and embedded in paraffin. Sectioned tissues were stained with H&E and with antibody against MCM7 for immunohistochemistry. Histological analysis was performed under a light microscope and the blood vessels are shown (arrow). Scale bars, 200 μm for H&E staining. 100 μm for MCM7 staining. ADR, Adriamycin; H&E, hematoxylin and eosin; MCM, minichromosome maintenance.
J Cancer Prev 2022;27:221~228
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