Fig. 3. Cedrol induces apoptosis in HT29 cells. (A) Annexin V/7-Aminoactinomycin D (7-AAD) double staining. Cells were treated with cedrol for 48 hours and double stained with Annexin V and 7-AAD. Dot blot and the percentage of live, total apoptotic and dead cells are shown. Live cells (lower left): Annexin V^-/7-AAD^-, dead cells (upper left): Annexin V-/7-AAD⁺, early apoptotic cells (lower right): Annexin V⁺/7-AAD^-, late apoptotic cells (upper right): Annexin V⁺/7-AAD⁺. (B) Distribution of cell population in each quadrant of the dot plots. Data are presented as the mean ± standard deviation of triplicate experiments. *P < 0.05, **P < 0.01 vs. control. (C) DAPI staining. Cedrol-treated cells were fixed and stained with DAPI. Arrows indicate the apoptotic bodies. Scale bars, 50 μm. (D) Modulation of apoptosis-related protein expression by cedrol in HT29 cells. Cells were treated with indicated concentrations of cedrol for 48 hours and western blotting was performed using apoptosis-related protein antibodies. Actin was used as an internal control. PARP, poly ADP ribose polymerase; DAPI, 4′,6-diamidino-2-phenylindol.

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