Journal of Cancer Prevention

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Fig. 1.

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Fig. 1. Cedrol inhibits cell growth and MCM protein expression in human adenocarcinoma HT29 cells. (A) Chemical of cedrol. (B) Inhibitory effects of cedrol on HT29 cell growth. Cells were treated with indicated concentrations of cedrol for 24 hours or 48 hours. Cell viability was measured by the water-soluble tetrazolium salt assay as described in Materials and Methods. Control cells were treated with 0.1% dimethylsulfoxide. All experiments were repeated three times, and the values and bars represented mean ± standard deviation, respectively. *P < 0.05, **P < 0.01 vs. control. (C) Inhbition of minichromosome maintenance by cedrol treatment in HT29 cells. Cells were treated with various concentrations of cedrol for 48 hours. The cells were lysed, and the cellular proteins were then separated by sulfate-polyacrylamide gel electrophoresis and transferred onto nitrocellulose membranes. The membranes were proved with the indicated antibodies and the proteins were visualized using the enhanced chemiluminescence detection system. Actin was used as an internal control. MCM, minichromosome maintenance; PCNA. proliferating cell nuclear antigen.
J Cancer Prev 2022;27:221~228 https://doi.org/10.15430/JCP.2022.27.4.221
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