Fig. 2. The activation of AMP-activated protein kinase α (AMPKα) by 6-(methylsulfinyl) hexyl isothiocyanate (6-MSITC). HepG2 cells were treated with 10 μM of 6-MSITC for 3, 6, 9, 12, 24, 36, and 48 hours (A) or with 5-20 μM of 6-MSITC for 6 hours (B). The whole-cell lysates were used to measure p-AMPKα and β-actin by Western blot analysis with their respective antibodies. Densitometry of the blots was performed using the LumiVision Imager software. Histograms show the densitometric analysis of p-AMPKα normalized to β-actin. The data were from triplicate tests. *P < 0.05, **P < 0.05.

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