Fig. 6. Mannose attenuated colitis-associated tumorigenesis by targeting TAMs in mice.
(A) F4/80 and CD206 in colon tissues were detected by immunohistochemical analysis. (B) The mRNA levels of TAMs markers (VEGF, Arg-1, and HIF-1α) in colon tissues of each group mice. (C) The protein levels of GLUT1, HK II, HIF-1α, Arg-1, and COX-2 in the colon tissues. Data represent the mean ± standard deviation. TAMs, tumor-associated macrophages; HK II, hexokinase II; GLUT1, glucose transporters 1; Arg-1, arginase-1; NAT, normal adjacent tissue; TT, tumor tissue; ns, not significant; AOM, azoxymethane; DSS, dextran sodium sulfate; IHC, immunohistochemical. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the AOM/DSS group.