Journal of Cancer Prevention

eISSN 2288-3657
pISSN 2288-3649
Fig. 5.

Download original image

Fig. 5. Mannose regulated TAM polarization by lactate in vitro. (A) Schematic diagram of method for construction of TAMs. (B) The mRNA levels of TAM markers (VEGF, Arg-1, and HIF-1α) in TAMs derived from THP-1 cells subjected to differentiation by PMA and subsequent stimulation with HCT116 cells. (C) Effect of lactate on the mRNA expression of VEGF, Arg-1, and HIF-1α during TAM polarization. THP-1 cells were differentiated into M0 macrophages by incubation with PMA for 24 hours. Then 1, 3, and 10 mmol/L lactate was treated to M0 THP-1 cells. Data represent the mean ± standard deviation. TAMs, tumor-associated macrophages; Arg-1, arginase-1; HIF-1α, hypoxia-inducible factor 1-alpha; PMA, phorbol 12-myristate 13-acetate. *P < 0.05, **P < 0.01 and ***P < 0.001.
J Cancer Prev 2022;27:31~41 https://doi.org/10.15430/JCP.2022.27.1.31
© J Cancer Prev
© Korean Society of Cancer Prevention. / Powered by INFOrang Co., Ltd