Fig. 6. Effect of DATS administration on mRNA and protein expression of neurofascin (NFASC) and N-acetyltransferase domain containing 1 (NATD1) in mammary tumors of rats. (A) Quantification of NFASC and NATD1 levels in RNA-seq data. Results shown are mean ± SD (n = 3) and analyzed by unpaired Student’s t-test (*P < 0.05). (B) Immunoblots for NFASC, NATD1, and β-actin in tumor lysates from control and DATS-treated rats. (C) Quantification of the expression of the proteins shown in (B). Results shown are mean ± SD (n = 6 for both groups). FPKM, the expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced; DATS, diallyl trisulfide.

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