Fig. 5. GGE03 inhibits Helicobacter pylori (H. pylori)-induced myeloperoxidase (MPO) activity and expression of IFN-γ in AGS cells. (A) AGS cells were pretreated with GE and GGE03 at indicated concentrations for 1 hour, followed by 1.5 hours co-culture with H. pylori (50 multiplicity of infection [MOI]). After incubation, the cell lysates were collected for determination of MPO activity as described in Materials and Methods. (B) AGS cells were pretreated with GE and GGE03 indicated concentrations for 1 hour, followed by 12 hours co-culture with H. pylori (50 MOI). The effect of GE and GGE03 on the expression of IFN-γ in AGS cells was analyzed by qRT-PCR. 18S rRNA was used as an internal control. Data are the mean ± SD. Statistical significance was analyzed by ANOVA. n.s., not significant. ***P < 0.001 compared to control; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared to the H. pylori group.

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