eISSN 2288-3657
pISSN 2288-3649
pISSN 2288-3649
Primer sequences used in RT-PCR and qRT-PCR primers
| Species | Gene | Primer sequence | |
|---|---|---|---|
| Human (RT-PCR) | 18S rRNA | Foward | CCCAACTTCTTAGAGGGACAAGT |
| Reverse | TAGTCAAGTTCGACCGTCTTCTC | ||
| iNOS | Foward | GGCCTCTCAGCTCACCCCGA | |
| Reverse | CCAGGCGCACTGTCTGGTGG | ||
| Human (qRT-PCR) | 18S rRNA | Foward | GCAATTATTCCCCATGAACG |
| Reverse | GGCCTCACTAAACCATCCAA | ||
| IL-8 | Foward | TCC TTG TTC CAC TGT GCC TTG | |
| Reverse | TGC TTC CAC ATG TCC TCA CAA | ||
| TNF-α | Foward | TCA GAG GGC CTG TAC CTC AT | |
| Reverse | GGA AGA CCC CTC CCA GAT AG | ||
| IL-6 | Foward | AGGGCTCTTCGGCAAATGTA | |
| Reverse | GAAGGAATGCCCATTAACAACAA | ||
| IL-1β | Foward | TTA AAG CCC GCC TGA CAG A | |
| Reverse | GCG AAT GAC AGA GGG TTT CTT | ||
| iNOS | Foward | AGGTCCAAATCTTGCCTGGG | |
| Reverse | ATCTGGAGGGGTAGGCTTGT | ||
| IFN-γ | Foward | ACTGTCGCCAGCAGCTAAAA | |
| Reverse | TATTGCAGGCAGGACAACCA | ||
RT-PCR, reverse transcription PCR; qRT-PCR, quantitative real-time PCR; iNOS, inducible NOS; IL, interleukin.
J Cancer Prev 2021;26:289~297 https://doi.org/10.15430/JCP.2021.26.4.289
© J Cancer Prev
© Korean Society of Cancer Prevention. / Powered by INFOrang Co., Ltd