Journal of Cancer Prevention

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Fig. 4.

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Fig. 4. Cysteine 259 residue of STAT3 as a putative binding site of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2). (A) Cysteine residues present in human STAT3. The STAT3 protein consists of 770 amino acids and is divided into 6 distinct functional domains: the N-terminal domain, coiled-coil domain, DNA-binding domain, a linker domain, Src homology 2 (SH2) domain, and C-terminal transactivation domain. (B) Covalent binding of 15d-PGJ2 to STAT3 as predicted by computational modeling. Schrodinger program was used in docking analysis for 15d-PGJ2 interaction with cysteine 251 and 259 of STAT3 as described in Materials and Methods. (C) PC-3 cells were transiently transfected with GFP-tagged WT STAT3 (STAT3WT) or GFP-tagged mutant STAT3 (STAT3C251A or STAT3C259A) followed by 15d-PGJ2 treatment for 24 hours. The cleaved PARP was detected by Western blot analysis. SH2, Src homology 2; TA, transactivation; DBD, DNA-binding domain; CC, coiled-coil domain; MMGB-SA ΔG, binding free energy calculated with Molecular Mechanics with Generalized Born and Surface Area.
J Cancer Prev 2021;26:207~217
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