Journal of Cancer Prevention

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Fig. 3.

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Fig. 3. Effect of DHA on TNF-α- or poly (I:C)-induced increase in intracellular and mitochondrial ROS levels in pancreatic stellate cells (PSCs). PSCs were pre-treated with 50 μM DHA for 1 hour, and then stimulated with TNF-α (10 ng/mL) or poly (I:C) (1 μg/mL) for 1 hour. (A) Intracellular ROS levels were determined by 5,6-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA) assay. (B) Mitochondrial ROS levels were measured using MitoSOX reagent (Invitrogen, Carlsbad, CA, USA). Data are expressed as the mean ± SE. ROS, reactive oxygen species; poly (I:C), polyinosinic-polycytidylic acid; DHA, docosahexaenoic acid. *P < 0.05 vs. untreated cells; P < 0.05 vs. cells treated with TNF-α or poly (I:C) alone.
J Cancer Prev 2021;26:195~206
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