Journal of Cancer Prevention

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Fig. 2.

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Fig. 2. DHA inhibits TNF-α- and poly (I:C)-induced expression of MCP-1 and CX3CL1 in pancreatic stellate cells (PSCs). (A, B) The cells were pre-treated with the indicated concentrations of DHA and stimulated with TNF-α (10 ng/mL) or poly (I:C) (1 μg/mL) for 3 hours. mRNA expression was determined by real-time PCR analysis and normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C, D) The cells were pre-treated with DHA (50 μM) and stimulated with TNF-α (10 ng/mL) or poly (I:C) (1 μg/mL) for 24 hours. Protein levels in the medium were determined by enzyme-linked immunosorbent assay (ELISA). Data are expressed as the mean ± SE. MCP-1, monocyte chemoattractant protein 1; poly (I:C), polyinosinic-polycytidylic acid; DHA, docosahexaenoic acid; CX3CL1, chemokine C-X3-C motif ligand 1. *P < 0.05 vs. untreated cells; P < 0.05 vs. cells treated with TNF-α or poly (I:C) alone.
J Cancer Prev 2021;26:195~206 https://doi.org/10.15430/JCP.2021.26.3.195
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