Journal of Cancer Prevention

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Fig. 3.

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Fig. 3. Identification of the characteristics of breast cancer stem-like cells induced to differentiate. (A, B) To establish mammospheres, corresponding cells were seeded in Ultra-Low six-well plates using cancer stem cells culture media for 21 days. Magnification, ×100. (C, D) CD24low/CD44+ population analysis was performed on day 0 and day 21 of differentiation induction. Representative flow cytometry dot plots of CD24 and CD44 expression of breast cancer stem-like cells. The square indicates the distribution of the CD24low/CD44+ cell subpopulation. (E, F) Representative blots showing the expression levels of OCT4 and proliferation cell nuclear antigen (PCNA) measured by Western blotting assays. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control.
J Cancer Prev 2021;26:183~194 https://doi.org/10.15430/JCP.2021.26.3.183
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