Journal of Cancer Prevention

eISSN 2288-3657
pISSN 2288-3649
Fig. 3.

Download original image

Fig. 3. Identification of the characteristics of breast cancer stem-like cells induced to differentiate. (A, B) To establish mammospheres, corresponding cells were seeded in Ultra-Low six-well plates using cancer stem cells culture media for 21 days. Magnification, ×100. (C, D) CD24low/CD44+ population analysis was performed on day 0 and day 21 of differentiation induction. Representative flow cytometry dot plots of CD24 and CD44 expression of breast cancer stem-like cells. The square indicates the distribution of the CD24low/CD44+ cell subpopulation. (E, F) Representative blots showing the expression levels of OCT4 and proliferation cell nuclear antigen (PCNA) measured by Western blotting assays. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control.
J Cancer Prev 2021;26:183~194
© J Cancer Prev
Copyright © Korean Society of Cancer Prevention. / Powered by INFOrang Co., Ltd