Fig. 2. Effect of dioscin on cell migration, invasion, and colony formation in breast cancer cells. (A, B) Effect of dioscin on the migration of breast cancer cells. The cells were grown to confluence in 6-well plates, scratch-wounded, and treated with the indicated concentrations of dioscin. Magnification, ×100. (C, D) Quantification of data shown in A and B. (E, F) Transwell assays were performed to determine the cell migration (without Matrigel) and invasion (with Matrigel) of the breast cancer cells exposed to dioscin. Magnification, ×100. (G, H) Quantification of data shown in E and F. (I, J) Effect of dioscin on the colony formation of breast cancer cells. The cancer cells were incubated in six-well plates and treated with dioscin. (K, L) Quantification of data shown in I and J. Magnification, ×100. NC, negative control. The values represent the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 compared to the controls.

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