Journal of Cancer Prevention

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Fig. 5.

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Fig. 5. PEBP elevates the expression of miR-200b in transfected B16F10 cells. Relative normalized expression (RT2-quantitative real-time PCR [qPCR] analysis) of miR-200b after 24-hour exposure to either PEBP or NBJ. B16F10 cells could grow to 30% confluence in DMEM medium. Cells were transfected with miR-200b mimic, anti-miR miRNA Inhibitor, control (NLNT), Lipofectamine no target (LNT) and noncoding RNA (NC1) using Lipofectamine 2000 (Invitrogen, Burlington, ON, Canada), and the expression of miR-200b was detected by RT2-qPCR. Two-Way ANOVA was used to analyze the statistical difference between the groups. Data represent a combination of 3 experiments. All data are presented as mean ± SEM. Significance shows as *P < 0.05, ***P < 0.001, ****P < 0.0001 different from control. PEBP, polyphenol enriched blueberry preparation; NBJ, non-fermented blueberry juice.
J Cancer Prev 2021;26:162~173 https://doi.org/10.15430/JCP.2021.26.3.162
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