Journal of Cancer Prevention

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pISSN 2288-3649
Fig. 4.

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Fig. 4. Expression of miR-200b is upregulated in skin cancer. Relative normalized expression (RT2-quantitative real-time PCR [qPCR] analysis) of miR-200b in B16F10 cells after 24 hours exposure to different concentrations of PEBP or NBJ. Cells were plated in (A) 6-well attachment plates in Dulbecco’s Modified Eagle’s Medium (DMEM) and (B) 6-well ultra-low attachment plates in DMEM-F12 (spheroid medium) and incubated at 5% CO2 and 37ºC. Data were normalized to the RNA control U6snRNA. One-way ANOVA, followed by post-hoc Tukey’s multiple comparisons were used. Data represent a combination of 3 experiments. All data are presented as mean ± SEM. Significance shows as **P < 0.01, ***P < 0.001 different from control. PEBP, polyphenol enriched blueberry preparation; NBJ, non-fermented blueberry juice.
J Cancer Prev 2021;26:162~173
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