Fig. 1. Sphere growth is inhibited after PEBP treatment.
Inhibition of human melanoma HS 294T (A) and murine melanoma B16F10 (B) spheroid development after treatment with 100 and 150 μM GAE (gallic acid equivalent) of either PEBP or NBJ. Photographs of HS294T (C) and B16F10 (D) spheres taken with AxioCamMR3 camera on light microscope (magnification, ×20). After 2 days in culture. Spheres are isolated and grown in 96-well plates at 37°C and 5% CO2. Significance shows as *P < 0.05, **P < 0.01, ****P < 0.0001 different from control. Two-Way ANOVA was used. Data represent a combination of 3 experiments. All data are presented as mean ± SEM. PEBP, polyphenol enriched blueberry preparation; NBJ, non-fermented blueberry juice.