Fig. 2. TFF1 suppresses migration and invasion in AGS cells. (A) AGS cells were transfected with TFF1 for 24 hours. After transfection, the cell migration was analyzed for 24 hours in scratched AGS cells. (B) Cell invasion was analyzed after 48 hours post transfection with TFF1 to allow for the permeabilization of the transwell membrane. The membrane was stained with 0.2% crystal violet. Scale bar, 100 mm. TFF1, Trefoil factor 1. *P < 0.05, **P < 0.01, and ***P < 0.001, significantly different compared with control.

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