Journal of Cancer Prevention

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Fig. 5.

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Fig. 5. Effect of DATS treatment on the expression of SLIT2/ROBO1 signaling-related proteins. Immunoblot analysis for SLIT2, ROBO1, CXCR4, and b-Actin proteins using lysates from MCF-7 and MDA-MB-231 cells treated with either DMSO (control) or desired doses of DATS for indicated time points. Numbers above bands are fold change of each protein relative to corresponding DMSO-treated control. Experiments were repeated twice with comparable results. Molecular weights for the observed bands for SLIT2 were indicated. DATS, diallyl trisulfide; MCF-7, michigan cancer foundation-7; MDA-MB-231, MD anderson-metastatic breast-231; CXCR4, C-X-C motif chemokine receptor 4; DMSO, dimethyl sulfoxide.
J Cancer Prev 2021;26:128~136
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