Journal of Cancer Prevention

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Fig. 1.

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Fig. 1. Heregulin-β1 stimulates the migration of MCF-7 cells and induces expression of manganese superoxide dismutase (MnSOD). (A) MCF-7 cells were treated with heregulin-β1 for 24 hours and subjected to the wound healing assay. (B) mRNA expression correlation data between neuregulin-1 (NRG1) and MnSOD in the metastatic breast cancer (TCGA provisional) were obtained by using the cBioportal web interface. (C) MCF-7 cells were treated with heregulin-β1 (50 ng/mL) for indicated time periods. The expression of MnSOD mRNA and protein was measured by RT-PCR and Western blot analyses, respectively as described in Materials and Methods. (D). MCF-7 cells were treated with 0, 1, 10, or 50 ng/mL of heregulin-β1 for 8 hours (for mRNA) and 24 hours (for protein). The expression of MnSOD mRNA and protein was measured by RT-PCR and Western blot analyses, respectively. (E) Immunocytochemical analysis was performed using an anti-MnSOD antibody after the treatment of MCF-7 cells with 50 ng/mL heregulin-β1 for 24 hours. Images of the cellular fluorescence were acquired using a confocal laser-scanning microscope as described under Materials and Methods; (a) Vehicle-control cells. (b) Cells treated with 50 ng/mL heregulin-β1 for 24 hours. Scale bar, 100 μm. (F) MCF-7 cells treated with heregulin-β1 (0, 1, 10, or 50 ng/mL) for 24 hours, and the catalytic activity of MnSOD was measured as described in Materials and Methods. *Significantly different from the control without heregulin-β1 treatment (P < 0.05).
J Cancer Prev 2021;26:54~63 https://doi.org/10.15430/JCP.2021.26.1.54
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