Fig. 4. Effect of piceatannol (PIC) on the expression of cell cycle-related proteins. Representative immunoblots showing the effect of PIC treatment on (A) LNCaP and (B) 22Rv1 cells. Lysates from both cell lines treated with dimethyl sulfoxide (DMSO) or indicated doses of PIC for 8, 16, and 24 hours were submitted to Western blotting for p53, p21, cyclin-dependent kinase 4 (cdk4), cyclin D1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and β-actin. The numbers on top of the band indicate fold change in protein level compared to corresponding DMSO-treated control. Housekeeping proteins were used for normalization (β-actin or GAPDH). The experiments were repeated at least twice with consistent results.

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